gene profiling analysis
Instructor: Chung-Jui Tsai (chtsai@mtu.edu), Professor, Phone: 487-2914
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Syllabus
1. Introduction slides
2. cDNA synthesis slides microarray
protocol
3. cDNA purification experimental design microarray protocol
[paper] Churchill
(2002) Fundamentals of experimental design for cDNA
microarrays. Nature Genetics 32S: 490-495
[paper] Cui and Churchill (2003)
How many mice and how many arrays? CAMDA
4. Cy dye coupling and target
purification data mining microarray protocol
5. Array hybridization microarray protocol
6. GenePix image acquisition GenePix manual microarray protocol (pages 9-11)
7. GeneSpring data analysis microarray protocol (pages 12-18)
8. Candidate gene selection and PCR
primer design real-time PCR
9. Quantitative RT-PCR SYBR
protocol
website of Dr. M. Tevfik Dorak The Reference in qPCR
Useful Links
GenBank, National
Center for Biotechnology Information
Joint Genome Institute
aspenDB
Yahoo! Groups: Microarray
Gene-Array
Discussion ListServ
GeneSpring Web Seminars
Integrated
DNA Technologies, Inc.
Complementary
Sequence Conversion
ClustalW
Multiple Sequence Alignment
ExPASy
Translate Tool
